[Abstract] Sepheo Ho fumana masala a N-terminal 15 amino acid a likaroloana tse peli tse tšoanang tsa Agacutin.Likarolo tse peli tsa molek'hule ea enzyme li hloka ho aroloa ho fumana ketane e le 'ngoe ea peptide le ho tatellano.Mekhoa ea Agacutin e hloekisitsoeng haholo e fumanoe ka mokhoa oa biochemical.Mehlala e le 'ngoe ea subunit bakeng sa ho tsebahatsa le tatellano e lokiselitsoe ke SDS-PAGE electrophoresis separation, subunit adhesive strip recovery le PVDF membrane electrotransfer technology.Ea ho qetela e ile ea etsa qeto ea masala a 15 a amino acid sebakeng sa N-terminal ea subunit ka mokhoa oa ho senyeha ha Edman.Liphetho Tatelano ea subunit e kholo (16 kDa) e ne e le DCSSGWSSYEEHQYY, le ea subunit e nyane (15 kDa) e ne e le DSSGWSSYEGHEYYV.Qetello Liphetho tsa tatellano li bonts'itse hore Agacutin e ne e le thrombin e ncha ea chefo ea noha joalo ka enzyme ka ho batlisisa database ea NCBI.
Nako ea poso: Dec-22-2022