Sepheo: Ho ithuta phello ea karolo ea "tumor suppression component I (AAVC-I)" mabapi le thibelo ea ho ata le apoptosis ea kankere ea matšoafo ea motho lisele tsa A549.Mekhoa: Mokhoa oa MTT o ne o sebelisetsoa ho lemoha litekanyetso tsa ho thibela 24h le 48h tsa lisele tsa A549 ka AAVC-I ka mekhahlelo e fapaneng.
HE staining le Hoechst33258 fluorescent staining li ile tsa sebelisoa ho hlokomela apoptosis ho tloha morphology;Immunohistochemistry e ne e sebelisetsoa ho bona liphetoho ho polelo ea protheine ea BAX.Liphello: MTT e bontšitse hore AAVC-I e ka thibela ho ata ha lisele tsa A549 ka mokhoa o itšetlehileng ka nako le tekanyo.Ka mor'a phekolo ea AAVCI bakeng sa 24h, thibelo ea nucleus, nyutlelie hyperstaining le lihlopha tsa apoptotic li ne li bonahala tlas'a microscopy.Immunohistochemistry e bonts'itse hore karolelano ea boleng ba optical density e eketsehile ka keketseho ea khatello ea lithethefatsi, e bonts'a hore polelo ea protheine ea BAX e ne e laoloa ka tsela e tšoanang.
Nako ea poso: May-11-2023